The isolation with cytidine diphosphate glycerol, cytidine diphosphate ribitol and mannitol 1-phosphate from Lactobacillus arabinosus

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Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol.

Kruh, J., Dreyfus, J. C. & Sohapira, G. (1960). J. biol. Chem. 286, 1075. Kruh, J., Dreyfus, J. C., Schapira, G. & Padieu, P. (1957). J. biol. Chem. 228, 113. Levy, A. L. (1954). Nature, Lond., 174, 126. Maisin, M. & Lindenberg, A. B. (1957). Bull. Soc. Chim. biol., Paris, 89, 78. Neuberger, A. (1951). In Ciba Foundation Conf. Isotopes in Biochemistry, p. 68. London: Churchill Ltd. Parnas, I. K...

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The Purification and Properties of Cytidine Diphosphate

Crude extracts of Salmonella fyphi catalyze the conversion of cytidine diphosphate D-glucose to CDP-tyvelose. Two intermediates in this reaction sequence have been identitied as CDP-4-keto-6-deoxyglucose and CDP-paratose. The enzyme catalyzing the conversion of CDP-D-glucose to CDP-4-keto-6-deoxyglucose (CDP-n-glucose oxidoreductase) has been purified approximately 50-fold from these extracts, ...

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Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. 3. Cytidine diphosphate D-glucose oxidoreductase.

A procedure is described for the purification of CDP-Dglucose oxidoreductase which catalyzes formation of CDP-4keto-6-deoxy-D-glucose and for enzymes El and ES which catalyze the further reduction to CDP-4-keto-3,6-dideoxy-Dglucose. All three enzymes have been purified to homogeneity as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. CDP-D-glucose oxidore...

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Enzymatic synthesis of cytidine diphosphate diglyceride.

Evidence is presented for the enzymatic formation of cytidine diphosphate diglyceride in microsomal preparations from guinea pig liver according to the reaction: CTP + phosphatidic acid right harpoon over left harpoon CDP-diglyceride + p-O-P. Conditions have been found in which the incorporation of labeled CTP into CDP-diglyceride is almost entirely dependent upon added phosphatidic acid. The i...

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Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower.

The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1956

ISSN: 0306-3283

DOI: 10.1042/bj0640599